abstract：:Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the h...

journal_title：DNA repair

authors： Charbonnel C,Allain E,Gallego ME,White CI

更新日期：2011-06-10 00:00:00

abstract：:Srs2 is a 3'-5' DNA helicase that regulates many aspects of DNA metabolism in Saccharomyces cerevisiae. It is best known for its ability to counteract homologous recombination by dismantling Rad51 filaments, but is also involved in checkpoint activation, adaptation and recovery, and in resolution of late recombination...

journal_title：DNA repair

authors： León Ortiz AM,Reid RJ,Dittmar JC,Rothstein R,Nicolas A

更新日期：2011-05-05 00:00:00

abstract：:During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...

journal_title：DNA repair

authors： Clark AB,Lujan SA,Kissling GE,Kunkel TA

更新日期：2011-05-05 00:00:00

abstract：:In both Schizosaccharomyces pombe and Saccharomyces cerevisiae, Mms22 and Mms1 form a complex with important functions in the response to DNA damage, loss of which leads to perturbations during replication. Furthermore, in S. cerevisiae, Mms1 has been suggested to function in concert with a Cullin-like protein, Rtt101...

journal_title：DNA repair

authors： Vejrup-Hansen R,Mizuno K,Miyabe I,Fleck O,Holmberg C,Murray JM,Carr AM,Nielsen O

更新日期：2011-03-07 00:00:00

abstract：:MutT enzymes prevent DNA damage by hydrolysis of 8-oxodGTP, an oxidized substrate for DNA synthesis and antimutagenic, anticarcinogenic, and antineurodegenerative functions of MutT enzymes are well established. MutT has been found in almost all kingdoms of life, including many bacterial species, yeasts, plants and mam...

journal_title：DNA repair

authors： Arczewska KD,Baumeier C,Kassahun H,Sengupta T,Bjørås M,Kuśmierek JT,Nilsen H

更新日期：2011-02-07 00:00:00

abstract：:The continuity of duplex DNA is generally considered a prerequisite for chromosome continuity. However, as previously shown in yeast as well as human cells, the introduction of a double-strand break (DSB) does not generate a chromosome break (CRB) in yeast or human cells. The transition from DSB to CRB was found to be...

journal_title：DNA repair

authors： Nakai W,Westmoreland J,Yeh E,Bloom K,Resnick MA

更新日期：2011-01-02 00:00:00

abstract：:The efficiency and fidelity of nucleotide incorporation and next-base extension by DNA polymerase (pol) κ past N(2)-ethyl-Gua were measured using steady-state and rapid kinetic analyses. DNA pol κ incorporated nucleotides and extended 3' termini opposite N(2)-ethyl-Gua with measured efficiencies and fidelities similar...

journal_title：DNA repair

authors： Pence MG,Blans P,Zink CN,Fishbein JC,Perrino FW

更新日期：2011-01-02 00:00:00

abstract：:Chemicals used industrially and commercially are required by law to be assessed for their genotoxic potential. However, all currently used assays have major limitations and despite intense effort, there is no universal agreement on which tests should be employed, or how to interpret results. We have developed a new as...

journal_title：DNA repair

authors： Evans TJ,Yamamoto KN,Hirota K,Takeda S

更新日期：2010-12-10 00:00:00

abstract：:Both UVB radiation and DNA-breaking agents were previously reported to kill Arabidopsis stem cells. We demonstrate that death induced by UVB or by ionizing radiation (IR) requires Suppressor of Gamma Response 1 (SOG1), a transcription factor already found to govern many responses to these agents in Arabidopsis. DNA-da...

journal_title：DNA repair

authors： Furukawa T,Curtis MJ,Tominey CM,Duong YH,Wilcox BW,Aggoune D,Hays JB,Britt AB

更新日期：2010-09-04 00:00:00

abstract：:Humans have five members of the well conserved RecQ helicase family: RecQ1, Bloom syndrome protein (BLM), Werner syndrome protein (WRN), RecQ4, and RecQ5, which are all known for their roles in maintaining genome stability. BLM, WRN, and RecQ4 are associated with premature aging and cancer predisposition. Of the three...

journal_title：DNA repair

authors： Rossi ML,Ghosh AK,Kulikowicz T,Croteau DL,Bohr VA

更新日期：2010-07-01 00:00:00

abstract：:Me-lex is a sequence-specific alkylating agent synthesized to preferentially (>90%) generate N3-methyladenine (3-mA) in the minor groove of double-strand DNA, in A-T rich regions. In this paper we investigated the effect of XRCC1 deficiency in the processing of 3-mA adducts generated by Me-lex, through the molecular a...

journal_title：DNA repair

authors： Russo D,Fronza G,Ottaggio L,Monti P,Perfumo C,Inga A,Iyer P,Gold B,Menichini P

更新日期：2010-07-01 00:00:00

abstract：:Human APOBEC3G (A3G) and activation-induced deaminase (AID) belong to a family of DNA-cytosine deaminases. While A3G targets the last C in a run of C's, AID targets C in the consensus sequence WRC (W is A or T and R is a purine). Guided by the structures of the A3G carboxyl-terminal catalytic domain (A3G-CTD), we iden...

journal_title：DNA repair

authors： Carpenter MA,Rajagurubandara E,Wijesinghe P,Bhagwat AS

更新日期：2010-05-04 00:00:00

abstract：:Through the action of multiple sensors, mediators, and effectors, the DNA damage response (DDR) orchestrates the repair of DNA damage to ensure maintenance of genomic integrity. Recently, in addition to phosphorylation, other post-translational modifications such as ubiquitylation and SUMOylation have emerged as impor...

journal_title：DNA repair

authors： Zlatanou A,Stewart GS

更新日期：2010-05-04 00:00:00

abstract：:Hydrolytic deamination of DNA cytosine residues results in U/G mispairs, pre-mutagenic lesions threatening long-term genetic stability. Hence, DNA uracil repair is ubiquitous throughout all extant life forms and base excision repair, triggered by a uracil DNA glycosylase (UDG), is the mechanistic paradigm adopted, as ...

journal_title：DNA repair

authors： Schomacher L,Schürer KA,Ciirdaeva E,McDermott P,Chong JP,Kramer W,Fritz HJ

更新日期：2010-04-04 00:00:00

abstract：:S(N)1-type methylating agents generate O(6)-methyl guanine (O(6)-meG), which is a potently mutagenic, toxic, and recombinogenic DNA adduct. Recognition of O(6)-meG:T mismatches by mismatch repair (MMR) causes sister chromatid exchanges, which are representative of homologous recombination (HR) events. Although the MMR...

journal_title：DNA repair

authors： Rajesh P,Rajesh C,Wyatt MD,Pittman DL

更新日期：2010-04-04 00:00:00

abstract：:The RecG protein of Escherichia coli is a double-stranded DNA translocase that unwinds a variety of branched DNAs in vitro, including Holliday junctions, replication forks, D-loops and R-loops. Coupled with the reported pleiotropy of recG mutations, this broad range of potential targets has made it hard to pin down wh...

journal_title：DNA repair

authors： Rudolph CJ,Upton AL,Briggs GS,Lloyd RG

更新日期：2010-03-02 00:00:00

abstract：:RECQ proteins are conserved DNA helicases in both prokaryotes and eukaryotes. The importance of the RECQ family helicases in human health is demonstrated by their roles as cancer suppressors that are vital for preserving genome integrity. Mutations in one of the RECQ family proteins, RECQ4, not only result in developm...

journal_title：DNA repair

authors： Liu Y

更新日期：2010-03-02 00:00:00

abstract：:PriA, a 3'-->5' superfamily 2 DNA helicase, acts to remodel stalled replication forks and as a specificity factor for origin-independent assembly of a new replisome at the stalled fork. The ability of PriA to initiate replication at stalled forked structures ensures complete genome replication and helps to protect the...

journal_title：DNA repair

authors： Gabbai CB,Marians KJ

更新日期：2010-03-02 00:00:00

abstract：:The RecQ family of helicases are traditionally viewed as recombination factors, important for maintaining genome stability. RECQL5 is unique among these proteins in being associated with RNA polymerase II, the enzyme responsible for transcribing all protein-encoding genes in eukaryotes. Here, we describe the possible ...

journal_title：DNA repair

authors： Aygün O,Svejstrup JQ

更新日期：2010-03-02 00:00:00

abstract：:We previously showed that Caenorhabditis elegans APN-1, the only metazoan apurinic/apyrimidinc (AP) endonuclease belonging to the endonuclease IV family, can functionally rescue the DNA repair defects of Saccharomyces cerevisiae mutants completely lacking AP endonuclease/3'-diesterase activities. While this complement...

journal_title：DNA repair

authors： Zakaria C,Kassahun H,Yang X,Labbé JC,Nilsen H,Ramotar D

更新日期：2010-02-04 00:00:00

abstract：:Bladder cancer risk is highly influenced by environmental and/or predisposing genetic factors. In the last decades growing evidence of the major role played by DNA repair systems in the developing of bladder cancer has been provided. To better investigate the involvement of DNA repair genes previously reported to be s...

journal_title：DNA repair

authors： Ricceri F,Guarrera S,Sacerdote C,Polidoro S,Allione A,Fontana D,Destefanis P,Tizzani A,Casetta G,Cucchiarale G,Vineis P,Matullo G

更新日期：2010-02-04 00:00:00

abstract：:The ubiquitin-proteasome pathway plays an important role in DNA damage signaling and repair by facilitating the recruitment and activation of DNA repair factors and signaling proteins at sites of damaged chromatin. Proteasome activity is generally not thought to be required for activation of apical signaling kinases i...

journal_title：DNA repair

authors： Sakasai R,Teraoka H,Tibbetts RS

更新日期：2010-01-02 00:00:00

abstract：:Current data indicate that clustered DNA damage generated by ionizing radiation contains 2-5 damages within 20 bps. The complexity of clustered damage is also believed to increase as the linear energy transfer of the radiation increases. Complex lesions are therefore biologically relevant especially with the use of ca...

journal_title：DNA repair

authors： Malyarchuk S,Castore R,Harrison L

更新日期：2009-12-03 00:00:00

abstract：:Mice defective for the Polk gene, which encodes DNA polymerase kappa, are viable and do not manifest obvious phenotypes. The present studies document a spontaneous mutator phenotype in Polk(-/-) mice. The initial indication of enhanced spontaneous mutations in these mice came from the serendipitous observation of a po...

journal_title：DNA repair

authors： Stancel JN,McDaniel LD,Velasco S,Richardson J,Guo C,Friedberg EC

更新日期：2009-12-03 00:00:00

abstract：:Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capa...

journal_title：DNA repair

authors： Akbari M,Peña-Diaz J,Andersen S,Liabakk NB,Otterlei M,Krokan HE

更新日期：2009-07-04 00:00:00

abstract：:In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...

journal_title：DNA repair

authors： Lingaraju GM,Prota AE,Winkler FK

更新日期：2009-07-04 00:00:00

abstract：:Chk1 is a protein kinase that acts as a key signal transducer within the complex network responsible of the cellular response to different DNA damages. It is a conserved element along the eukaryotic kingdom, together with a second checkpoint kinase, called Chk2/Rad53. In fact, all organisms studied so far carried at l...

journal_title：DNA repair

authors： Pérez-Martín J

更新日期：2009-06-04 00:00:00

abstract：:Maintenance of the mitochondrial genome (mtDNA) is essential for proper cellular function. The accumulation of damage and mutations in the mtDNA leads to diseases, cancer, and aging. Mammalian mitochondria have proficient base excision repair, but the existence of other DNA repair pathways is still unclear. Deficienci...

journal_title：DNA repair

authors： de Souza-Pinto NC,Mason PA,Hashiguchi K,Weissman L,Tian J,Guay D,Lebel M,Stevnsner TV,Rasmussen LJ,Bohr VA

更新日期：2009-06-04 00:00:00

abstract：:Deinococcus radiodurans survives extremely high doses of ionizing and ultraviolet radiation and treatment with various DNA-damaging chemicals. As an effort to identify and characterize proteins that function in DNA repair in this organism, we have studied the protein encoded by locus DR1572. This gene is predicted to ...

journal_title：DNA repair

authors： Cao Z,Julin DA

更新日期：2009-05-01 00:00:00

abstract：:Eukaryotic cells have to regulate the progression and integrity of DNA replication forks through concomitantly transcribed genes. A transcription-dependent increase of recombination within protein-coding and ribosomal genes of eukaryotic cells is well documented. Here we addressed whether tRNA transcription and tRNA-d...

journal_title：DNA repair

authors： de la Loza MC,Wellinger RE,Aguilera A

更新日期：2009-05-01 00:00:00

abstract：:Eukaryotic cells repair ultraviolet light (UV)- and chemical carcinogen-induced DNA strand-distorting damage through the nucleotide excision repair (NER) pathway. Concurrent activation of the DNA damage checkpoints is also required to arrest the cell cycle and allow time for NER action. Recent studies uncovered critic...

journal_title：DNA repair

authors： Hannah J,Zhou P

更新日期：2009-04-05 00:00:00

abstract：:Ubiquitin conjugation plays critical roles in virtually all DNA repair pathways. This review provides an overview of the known multi-domain RING/Ubox E3 ligases and their domain structures. An analysis of known RING/Ubox X-ray and NMR structures leads to a discussion of the effects of dimerization. Structural and mech...

journal_title：DNA repair

authors： Hibbert RG,Mattiroli F,Sixma TK

更新日期：2009-04-05 00:00:00

abstract：:The proximity of the mitochondrial genome to the respiratory chain, a major source of ROS (radical oxygen species), makes mtDNA more vulnerable to oxidative damage than nuclear DNA. Mitochondrial BER (base excision repair) is generally considered to be the main pathway involved in the prevention of oxidative lesion-in...

journal_title：DNA repair

authors： Kaniak A,Dzierzbicki P,Rogowska AT,Malc E,Fikus M,Ciesla Z

更新日期：2009-03-01 00:00:00

abstract：:Several types of DNA lesion are induced after ionizing irradiation (IR) of which double strand breaks (DSBs) are expected to be the most lethal, although single strand breaks (SSBs) and DNA base damages are quantitatively in the majority. Proteins of the base excision repair (BER) pathway repair these numerous lesions...

journal_title：DNA repair

authors： Neijenhuis S,Verwijs-Janssen M,Kasten-Pisula U,Rumping G,Borgmann K,Dikomey E,Begg AC,Vens C

更新日期：2009-03-01 00:00:00

abstract：:The Karpas-620 human myeloma cell line (HMCL) expresses high levels of Cyclin D1 (CCND1), but has a der(8)t(8;11) and a der(14)t(8;14), and not a conventional t(11;14). Fluorescent in situ hybridization (FISH) and array comparative genomic hybridization (aCGH) studies suggest that der(14)t(11;14) from a primary transl...

journal_title：DNA repair

authors： Dib A,Glebov OK,Shou Y,Singer RH,Kuehl WM

更新日期：2009-03-01 00:00:00

abstract：:RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA ...

journal_title：DNA repair

authors： Nakayama M,Yamaguchi S,Sagisu Y,Sakurai H,Ito F,Kawasaki K

更新日期：2009-02-01 00:00:00

abstract：:Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells...

journal_title：DNA repair

authors： Salzano A,Kochiashvili N,Nergadze SG,Khoriauli L,Smirnova A,Ruiz-Herrera A,Mondello C,Giulotto E

更新日期：2009-01-01 00:00:00

abstract：:The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...

journal_title：DNA repair

authors： Sunavala-Dossabhoy G,De Benedetti A

更新日期：2009-01-01 00:00:00

abstract：:We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients show growth retardation, immature sexual development, and neurological ...

journal_title：DNA repair

authors： Nakane H,Hirota S,Brooks PJ,Nakabeppu Y,Nakatsu Y,Nishimune Y,Iino A,Tanaka K

更新日期：2008-12-01 00:00:00

abstract：:DNA polymerases play a crucial role in the cell cycle due to their involvement in genome replication and repair. Understanding the reaction mechanism by which these polymerases carry out their function can provide insights into these processes. Recently, the crystal structures of human DNA polymerase lambda (Pollambda...

journal_title：DNA repair

authors： Cisneros GA,Perera L,García-Díaz M,Bebenek K,Kunkel TA,Pedersen LG

更新日期：2008-11-01 00:00:00